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Light acclimation and pH perturbations affect photosynthetic performance in Chlorella mass culture
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SYSNO ASEP 0441494 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Light acclimation and pH perturbations affect photosynthetic performance in Chlorella mass culture Author(s) Ihnken, S. (NL)
Beardall, J. (AU)
Kromkamp, J.C. (NL)
Serrano, C.G. (ES)
Torres, M.A. (BR)
Masojídek, Jiří (MBU-M) RID, ORCID
Malapartida, I. (ES)
Abdala, R. (ES)
Jerez, C.G. (ES)
Malapascua, José R.F. (MBU-M)
Navarro, E. (ES)
Rico, R.M. (ES)
Peralta, E. (ES)
Ferreira Ezequil, J.P. (PT)
Figueroa, F.L. (ES)Number of authors 15 Source Title Aquatic Biology - ISSN 1864-7790
Roč. 22, č. 2 (2014), s. 95-110Number of pages 15 s. Language eng - English Country DE - Germany Keywords Chlorella ; Mass culture ; pH ; Chlorophyll fluorescence Subject RIV EE - Microbiology, Virology Institutional support MBU-M - RVO:61388971 UT WOS 000345701900008 Annotation Chlorella spp. are robust chlorophyte microalgal species frequently used in mass culture. The pH optimum for growth is close to neutrality; at this pH, theoretically little energy is required to maintain homeostasis. In the present study, we grew Chlorella fusca cells in an open, outdoor, thin-layer cascade photobioreactor (TLC), under ambient photon flux at the theoretically preferred pH (7.2), and let the culture pass the exponential growth phase. Using pH drift experiments, we show that an alkalization to pH 9 supported photosynthesis in the TLC. The increased photosynthetic activity under alkaline conditions was a pH-dependent effect, and not a dissolved inorganic carbon (DIC) concentration-or light intensity-dependent effect. Re-acidification (in one step or in increments) lowered gross oxygen production and increased non-photochemical quenching in short-term experiments. Gross oxygen production and electron transport rates in PSII were uncoupled during the pH perturbation experiments. Electron transport rates were only marginally affected by pH, whereas oxygen production rates decreased with acidification. Alternative electron pathways, electron donation at the plastid terminal oxidase and state-transitions are discussed as a potential explanation. Because cell material from the TLC was not operating at maximal capacity, we propose that alkalization can support photosynthesis in challenged TLC systems. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2015
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