Quadruplex-forming sequences occupy discrete regions inside plant LTR retrotransposons

Lexa, M.; Kejnovský, Eduard; Šteflová, Pavlína; Konvalinová, H.; Vorlíčková, Michaela; Vyskot, Boris

doi:https://dx.doi.org/10.1093/nar/gkt893

Number of the records: 1

Quadruplex-forming sequences occupy discrete regions inside plant LTR retrotransposons

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SYSNO ASEP	0440128
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Quadruplex-forming sequences occupy discrete regions inside plant LTR retrotransposons
Author(s)	Lexa, M. (CZ) Kejnovský, Eduard (BFU-R)_{RID, ORCID} Šteflová, Pavlína (BFU-R) Konvalinová, H. (CZ) Vorlíčková, Michaela (BFU-R)_{RID, ORCID} Vyskot, Boris (BFU-R)_{RID, ORCID}
Number of authors	6
Source Title	Nucleic Acids Research. - : Oxford University Press - ISSN 0305-1048 Roč. 42, č. 2 (2014), s. 968-978
Number of pages	11 s.
Publication form	Print - P
Language	eng - English
Country	GB - United Kingdom
Keywords	INTRAMOLECULAR DNA QUADRUPLEXES ; VIRUS TYPE-1 RNA ; CIRCULAR-DICHROISM
Subject RIV	BO - Biophysics
R&D Projects	GAP205/12/0466 GA ČR - Czech Science Foundation (CSF)
	GAP305/10/0930 GA ČR - Czech Science Foundation (CSF)
	GAP501/10/0102 GA ČR - Czech Science Foundation (CSF)
	GA522/09/0083 GA ČR - Czech Science Foundation (CSF)
	GPP501/10/P483 GA ČR - Czech Science Foundation (CSF)
Institutional support	BFU-R - RVO:68081707
UT WOS	000331138100030
DOI	10.1093/nar/gkt893
Annotation	Retrotransposons with long terminal repeats (LTR) form a significant proportion of eukaryotic genomes, especially in plants. They have gag and pol genes and several regulatory regions necessary for transcription and reverse transcription. We searched for potential quadruplex-forming sequences (PQSs) and potential triplex-forming sequences (PTSs) in 18 377 full-length LTR retrotransposons collected from 21 plant species. We found that PQSs were often located in LTRs, both upstream and downstream of promoters from which the whole retrotransposon is transcribed. Upstream-located guanine PQSs were dominant in the minus DNA strand, whereas downstream-located guanine PQSs prevailed in the plus strand, indicating their role both at transcriptional and post-transcriptional levels. Our circular dichroism spectroscopy measurements confirmed that these PQSs readily adopted guanine quadruplex structures-some of them were paralell-stranded, while others were anti-parallel-stranded.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2015

Number of the records: 1