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Sensitive detection of interferon-gamma with engineered proteins and surface plasmon resonance biosensor
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SYSNO ASEP 0437424 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Sensitive detection of interferon-gamma with engineered proteins and surface plasmon resonance biosensor Author(s) Šípová, Hana (URE-Y)
Ševců, Veronika (URE-Y)
Kuchař, Milan (BTO-N) RID
Ahmad, Jawid Nazir (MBU-M) RID
Mikulecký, Pavel (BTO-N) RID
Šebo, Peter (BTO-N)
Malý, Petr (BTO-N) RID, ORCID
Homola, Jiří (URE-Y) RIDNumber of authors 8 Source Title Procedia Engineering, 25 - EUROSENSORS XXV. - Amsterdam : Elsevier, 2011 / Tsamis Ch. ; Kaltsas G. - ISSN 1877-7058 - ISBN 978-1-62748-581-4 Pages s. 940-943 Number of pages 4 s. Publication form Print - P Action 25th Eurosensors Conference Event date 04.09.2011-07.09.2011 VEvent location Athens Country GR - Greece Event type WRD Language eng - English Country NL - Netherlands Keywords Surface plasmon resonance ; Biosensor ; Interferon gamma Subject RIV JA - Electronics ; Optoelectronics, Electrical Engineering Subject RIV - cooperation Institute of Biotechnology - Biotechnology ; Bionics
Institute of Microbiology - BiochemistryR&D Projects KAN200670701 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) KAN200520702 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) OC09058 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z20670512 - URE-Y (2005-2011) AV0Z50520701 - BTO-N (2007-2013) AV0Z50200510 - MBU-M (2005-2011) UT WOS 000300512400228 EID SCOPUS 84857185310 DOI 10.1016/j.proeng.2011.12.231 Annotation We present a novel surface plasmon resonance (SPR) biosensor for detection of human interferon gamma (hIFNy), an important diagnostic marker for several infectious diseases. The biosensor employs engineered proteins derived from albumin binding domain (ABD) and selected with ribosome display to provide high affinity towards hIFNy. We demonstrate that the biosensor provides rapid, sensitive, and cost-effective platform for the detection of hIFNγ and allows its detection at nanomolar levels both in buffer and diluted human plasma Workplace Institute of Radio Engineering and Electronics Contact Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Year of Publishing 2015
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