The X Chromosome Is Necessary for Somatic Development in the Dioecious Silene latifolia: Cytogenetic and Molecular Evidence and Sequencing of a Haploid Genome

Soukupová, Magda; Nevrtalová, Eva; Čížková, Jana; Vogel, Ivan; Čegan, Radim; Hobza, Roman; Vyskot, Boris

doi:https://dx.doi.org/10.1159/000363431

Number of the records: 1

The X Chromosome Is Necessary for Somatic Development in the Dioecious Silene latifolia: Cytogenetic and Molecular Evidence and Sequencing of a Haploid Genome

1.

SYSNO ASEP	0436672
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	The X Chromosome Is Necessary for Somatic Development in the Dioecious Silene latifolia: Cytogenetic and Molecular Evidence and Sequencing of a Haploid Genome
Author(s)	Soukupová, Magda (BFU-R) Nevrtalová, Eva (BFU-R) Čížková, Jana (UEB-Q)_{RID, ORCID} Vogel, Ivan (BFU-R) Čegan, Radim (BFU-R)_{RID, ORCID} Hobza, Roman (BFU-R)_{RID, ORCID} Vyskot, Boris (BFU-R)_{RID, ORCID}
Number of authors	7
Source Title	Cytogenetic and Genome Research. - : Karger - ISSN 1424-8581 Roč. 143, 1-3 (2014), s. 96-103
Number of pages	8 s.
Publication form	Print - P
Language	eng - English
Country	CH - Switzerland
Keywords	Anther culture ; Cytometry ; Dihaploids
Subject RIV	BO - Biophysics
Subject RIV - cooperation	Institute of Experimental Botany - Botanics
R&D Projects	GBP501/12/G090 GA ČR - Czech Science Foundation (CSF)
	GAP501/12/2220 GA ČR - Czech Science Foundation (CSF)
	GAP501/10/0102 GA ČR - Czech Science Foundation (CSF)
	LM2010005 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	BFU-R - RVO:68081707
UT WOS	000342614100010
DOI	10.1159/000363431
Annotation	Silene latifolia (or white campion) possesses a well-established sex determination system with a dominant Y chromosome in males (the mammalian type). The heteromorphic sex chromosomes X and Y in S. latifolia largely stopped recombination; thus, we can expect a gradual genetic degeneration of the Y chromosome. It is well proven that neither diploid nor polyploid S. latifolia sporophytes can survive without at least one X, so the only life stage possessing the Y as the sole sex chromosome is the male gametophyte (pollen tube), while the female gametophyte seems to be X-dependent. Previous studies on anther-derived plants of this species showed that the obtained plants (largely haploid or dihaploid) were phenotypically and cytologically female. In this paper, we provide molecular evidence for the inviability of plants lacking the X chromosome. Using sex-specific PCR primers, we show that all plantlets and plants derived from anther cultures are female. In studying anther-derived diploid females by sequencing of X-linked markers, we demonstrate that these plants are really homozygous dihaploids. A haploid regenerant plant was sequenced (8x genome coverage) using Illumina technology.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2015

Number of the records: 1