Molecular Plant Taxonomy: Methods and protocols

0436267 - BÚ 2015 RIV US eng M - Monography Chapter
Záveská Drábková, Lenka
DNA extraction from herbarium specimens.
Molecular Plant Taxonomy: Methods and protocols. vol.1115. New York: Springer Science+Business Media, 2014 - (Besse, P.), s. 67-84. Methods in Molecular Biology. ISBN 978-1-62703-766-2
R&D Projects: GA ČR(CZ) GAP506/11/0774; GA ČR GP206/07/P147
Institutional support: RVO:67985939
Keywords : DNA * herbarium speciemen * plants
Subject RIV: EF - Botanics

With the expansion of molecular techniques, the historical collections have become widely used. Studying plant DNA using modern molecular techniques such as DNA sequencing plays an important role in understanding evolutionary relationships, identification through DNA barcoding, conservation status and many other aspects of plant biology. Enormous herbarium collections are an important source of material especially for specimens from areas difficult to access or from taxa that are now extinct. The ability to utilize these specimens greatly enhances the research. However the process of extracting DNA from herbarium specimens is often fraught with difficulty related to such variables as plant chemistry, drying method of the specimen and chemical treatment of the specimen. Although many methods have been developed for extraction of DNA from herbarium specimens, the most frequently used are modified CTAB and DNeasy Plant Mini Kit protocols. Nine selected protocols have been described.
Permanent Link: http://hdl.handle.net/11104/0240029