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MICROTUBULE-SEVERING ATPASE KATANIN REGULATES CELL MOTILITY AND PROLIFERATION IN HUMAN GLIOBLASTOMA CELLS
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SYSNO ASEP 0431742 Document Type A - Abstract R&D Document Type The record was not marked in the RIV R&D Document Type Není vybrán druh dokumentu Title MICROTUBULE-SEVERING ATPASE KATANIN REGULATES CELL MOTILITY AND PROLIFERATION IN HUMAN GLIOBLASTOMA CELLS Author(s) Uhlířová, Jana (UMG-J)
Dráberová, Eduarda (UMG-J) RID, ORCID
Sládková, Vladimíra (UMG-J)
Dráber, Pavel (UMG-J) RID, ORCIDSource Title Cytoskeletal club 2014. - Vranovská Ves, 2014 Number of pages 1 s. Action Cytoskeletal club 2014 Event date 21.05.2014-23.5.2014 VEvent location Vranovská Ves Country CZ - Czech Republic Event type EUR Language eng - English Country CZ - Czech Republic Subject RIV EB - Genetics ; Molecular Biology R&D Projects LH12050 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) NT14467 GA MZd - Ministry of Health (MZ) Institutional support UMG-J - RVO:68378050 Annotation Glioblastoma multiforme is the most common, aggressive and chemorefraktory brain tumor in human adults. Microtubule-severing ATPases spastin, katanin and fidgetin are important regulators of microtubule organization. We have shown previously, that spastin is ovexpressed in gliomas and its depletion in human glioblastoma cell line T98G caused decrease of cell motility and increase in cell proliferation (J. Neuropathol. Exp. Neurol. 70:81, 2011). Here we report on expression, localization and function of katanin subunits p60 (catalytic) and p80 (regulatory) in human glioblastoma cell lines. RT-qPCR revealed differential expression of katanin subunits in glioblastoma cell lines. Both subunits localized to centrosomes, mitotic poles and leading edges of T98G cells. Free cell migration and cell morphology was changed in katanin-depleted T98G cells. We also observed changes in cell proliferation in katanin depleted cells. Collectively, our data indicates for the first time that katanin can regulate cell migration and proliferation in glioblastoma cells. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2015
Number of the records: 1