Recent Results in Cancer Research

0428990 - ÚJF 2015 RIV DE eng M - Monography Chapter
Eigner, Sebastian - Beckford, Denis R. - Fellner, M. - Loktionova, N. - Piel, M. - Melichar, František - Rosch, F. - Ross, T. L. - Lebeda, Ondřej - Eigner-Henke, Kateřina
Measurement of Protein Synthesis: In Vitro Comparison of 68Ga-DOTA-Puromycin, [3H]Tyrosine, and 2-Fluoro-[3H]tyrosine.
Recent Results in Cancer Research. Berlin: Springer, 2013 - (Baum, R.), s. 269-283. A Pathway to Personalised Diagnosis and Treatment. ISBN 978-3-642-27993-5
R&D Projects: GA MŠMT 2B06165; GA ČR(CZ) GA203/09/1056
Institutional support: RVO:61389005
Keywords : puromycine * Ga-68 * molecular imaging * PET * proteosynthesis
Subject RIV: FR - Pharmacology ; Medidal Chemistry

Puromycin has played an important role in our understanding of the eukaryotic ribosome and protein synthesis. It has been known for more than 40 years that this antibiotic is a universal protein synthesis inhibitor that acts as a structural analog of an aminoacyl-transfer RNA (aa-tRNA) in eukaryotic ribosomes. Due to the role of enzymes and their synthesis in situations of need (DNA damage, e.g., after chemo- or radiation therapy), determination of protein synthesis is important for control of antitumor therapy, to enhance long-term survival of tumor patients, and to minimize side-effects of therapy. Multiple attempts to reach this goal have been made through the last decades, mostly using radiolabeled amino acids, with limited or unsatisfactory success. The aim of this study is to estimate the possibility of determining protein synthesis ratios by using 68Ga-DOTA-puromycin (68Ga-DOTA-Pur), [3H]tyrosine, and 2-fluoro-[3H]tyrosine and to estimate the possibility of different pathways due to the fluorination of tyrosine.
Permanent Link: http://hdl.handle.net/11104/0234201