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Ultrastructure of Ehrlichia mineirensis, a new member of the Ehrlichia genus
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SYSNO ASEP 0423832 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Ultrastructure of Ehrlichia mineirensis, a new member of the Ehrlichia genus Author(s) Cabezas Cruz, Alejandro (BC-A)
Vancová, Marie (BC-A) RID, ORCID
Zweygarth, E. (DE)
Ribeiro, M.F.B. (BR)
Grubhoffer, Libor (BC-A) RID, ORCID
Passos, L.M.F. (BR)Source Title Veterinary Microbiology. - : Elsevier - ISSN 0378-1135
Roč. 167, 3-4 (2013), s. 455-458Number of pages 4 s. Publication form Print - P Language eng - English Country NL - Netherlands Keywords Ehrlichia mineirensis ; elctron microscopy ; in vitro culture Subject RIV EA - Cell Biology R&D Projects TE01020118 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) Institutional support BC-A - RVO:60077344 UT WOS 000330488600026 DOI 10.1016/j.vetmic.2013.08.001 Annotation Recently, we reported the in vitro isolation and the molecular characterization of a new species of Ehrlichia (Ehrlichia mineirensis) from haemolymph of Brazilian Rhipicephalus (Boophilus) microplus ticks. This organism shows an ortholog of Ehrlichia canis major immunogenic protein gp36 with a new structure of tandem repeats. In the present study, we used electron microscopy (high pressure freezing and freeze substitution preparative techniques) to characterize morphologically this new agent growing in IDE8 tick cells. The results showed that E. mineirensis shares ultrastructural features with other members of the genus Ehrlichia (Ehrlichia muris, E. canis and Ehrlichia chaffeensis); typical parasitophorous vacuoles (morulae) contain electron-dense and reticulated Ehrlichiae embedded inside a fibrillar matrix. We observed the characteristic Gram-negative-type cell wall composed of both cytoplasmic and rippled outer membrane. We found organisms undergoing binary fission and rarely altered cells with unusual invagination of the cytoplasmic membrane. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2014
Number of the records: 1