Characterization of Neutral Lipase BT-1 Isolated from the Labial Gland of Bombus terrestris Males

0421832 - ÚOCHB 2014 RIV US eng J - Journal Article
Brabcová, Jana - Prchalová, Darina - Demianova, Zuzana - Bučánková, A. - Vogel, H. - Valterová, Irena - Pichová, Iva - Zarevúcka, Marie
Characterization of Neutral Lipase BT-1 Isolated from the Labial Gland of Bombus terrestris Males.
PLoS ONE. Roč. 8, č. 11 (2013), e80066/1-e80066/11. ISSN 1932-6203. E-ISSN 1932-6203
R&D Projects: GA TA ČR TA01020969; GA ČR GA203/09/1446
Institutional support: RVO:61388963
Keywords : lipase * labial gland * Bombus terrestris
Subject RIV: CC - Organic Chemistry
Impact factor: 3.534, year: 2013
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0080066

This is the first report on neutral lipasefrom the cephalic part of B. terrestris labial glands. Purified lipase BT-1 displays broad specificity but preferentially hydrolyzes shorter fatty acid chains (C8-C12) from diacylglycerols and triacylglycerols. The lipase is most active at neutral pH, with a pH optimum of 8.3. The highest level of lipase BT-1 gene and protein expression is in young individuals. In bumblebees older than 3 days, the lipase gene expression level and enzymatic activity are decreased. Interestingly, according to transcript-level RNA seq analysis, the expression of lipase BT-1 is significantly higher in the bumblebee labial gland than in the fat body. The lipase BT-1 gene is the second most abundantly expressed gene in the labial gland of B. terrestris, which suggests important role of this enzyme in this tissue where pheromones are accumulated.
Permanent Link: http://hdl.handle.net/11104/0228080