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Interactions of γ-tubulin with GIT-PIX signaling cassette in mast cells
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SYSNO ASEP 0399691 Document Type A - Abstract R&D Document Type The record was not marked in the RIV R&D Document Type Není vybrán druh dokumentu Title Interactions of γ-tubulin with GIT-PIX signaling cassette in mast cells Author(s) Sulimenko, Vadym (UMG-J) RID, ORCID
Hájková, Zuzana (UMG-J)
Sulimenko, Tetyana (UMG-J)
Černohorská, Markéta (UMG-J)
Dráber, Pavel (UMG-J) RID, ORCIDSource Title Cytoskeletal club 2013. - Praha : Československá biologická společnost, 2013 Number of pages 1 s. Action Cytoskeletal Club 2013 Event date 15.05.2013-17.05.2013 VEvent location Vranovská Ves Country CZ - Czech Republic Event type EUR Language eng - English Country CZ - Czech Republic Keywords GIT-PIX signaling cassette ; Gama-tubulin ; Mast cell Subject RIV EB - Genetics ; Molecular Biology R&D Projects LD13015 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GPP302/11/P709 GA ČR - Czech Science Foundation (CSF) Institutional support UMG-J - RVO:68378050 Annotation The lecture presented our recent data of the interaction between γ-tubulin and proteins GIT and PIX in the course of mast cell activation. In general, rapid reorganization of microtubule cytoskeleton during mast cell activation is pivotal as well as signal transduction from surface receptors to centrosome, where microtubules are nucleated. γ-Tubulin is a key regulator of microtubule nucleation. Previously we have shown that γ-tubulin interacts with transiently phosphorylated proteins, some of them were identified as proteins GIT and PIX. These proteins are known to form stable complex called GIT-PIX signaling cassette. Pull-down experiments with truncated forms of γ-tubulin revealed that GIT and PIX proteins interact with the H11-H12 region of the C-terminal part of γ-tubulin. Moreover, these interactions are modulated by calcium ions concentration and by state of microtubule organization. These data suggest new interaction partners on the issue of γ-tubulin regulation in mast cells. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2014
Number of the records: 1