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Cytotoxic Constituents of Pachyrhizus tuberosus from Peruvian Amazon
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SYSNO ASEP 0399185 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Cytotoxic Constituents of Pachyrhizus tuberosus from Peruvian Amazon Author(s) Leuner, O. (CZ)
Havlík, J. (CZ)
Buděšínský, Miloš (UOCHB-X) RID, ORCID
Vrkoslav, Vladimír (UOCHB-X) RID, ORCID
Chu, J. (GB)
Bradshaw, T. D. (GB)
Hummelová, J. (CZ)
Mikšátková, P. (CZ)
Lapčík, O. (CZ)
Valterová, Irena (UOCHB-X) RID, ORCID
Kokoška, L. (CZ)Number of authors 11 Source Title Natural Product Communications - ISSN 1934-578X
Roč. 8, č. 10 (2013), s. 1423-1426Number of pages 4 s. Language eng - English Country US - United States Keywords Pachyrrhizus sp. ; Amazonian yam bean ; isoflavonoid ; rotenoid Subject RIV CC - Organic Chemistry Institutional support UOCHB-X - RVO:61388963 UT WOS 000326127900022 EID SCOPUS 84886803455 Annotation Investigations into the chemical constituents of the seeds of the neglected tuber crop Pachyrhizus tuberosus (Leguminosae) resulted in the isolation of seven components: five rotenoids [12a-hydroxyerosone (1), 12a-hydroxydolineone (2), erosone (3), 12a-hydroxyrotenone (4) and rotenone (6)], a phenylfuranocoumarin [pachyrrhizine (5)] and an isoflavanone [neotenone (7)]. The compounds were isolated using several chromatography techniques and characterized and verified by NMR and HPLC/MS. The MTT assay was used to examine the selective cytotoxic effects of the methanolic P. tuberosus extract and isolated compounds in two human cancer cell lines [breast (MCF-7) and colorectal (HCT-116)] and in non-transformed human fibroblasts (MRC-5); IC50 values were calculated. The methanolic P. tuberosus extract displayed respectable cytotoxic effects against HCT-116 and MCF-7 cells with IC50 values of 7.3 and 6.3 mu g/mL, respectively. Of the compounds, 6 exacted greatest cytotoxicity and selectivity towards the cancer cell lines tested, yielding IC50 values of 0.3 mu g/mL against both MCF-7 and HCT-116 cells, and a 6-fold reduced activity against MRC-5 fibroblasts. Compound 4 also demonstrated cytotoxicity against MCF-7 and HCT-116 (1.1 and 1.8 mu g/mL, respectively), and reduced cytotoxicity towards MRC-5 cells (7.5 mu g/mL). The results revealed from the in vitro cytotoxic MTT assay are worthy of further antitumor investigation. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2014
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