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Analytical isotachophoresis of lactate in human serum using dry film photoresist microfluidic chips compatible with a commercially available field-deployable instrument platform
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SYSNO ASEP 0398860 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Analytical isotachophoresis of lactate in human serum using dry film photoresist microfluidic chips compatible with a commercially available field-deployable instrument platform Author(s) Smejkal, P. (AU)
Breadmore, M. C. (AU)
Guijt, R. M. (AU)
Foret, František (UIACH-O) RID, ORCID
Bek, F. (DE)
Macka, M. (AU)Number of authors 6 Source Title Analytica Chimica Acta. - : Elsevier - ISSN 0003-2670
Roč. 803, NOV (2013), s. 135-142Number of pages 8 s. Publication form Print - P Language eng - English Country NL - Netherlands Keywords isotachophoresis ; microfluidics ; fluorescence ; indirect detection ; serum Subject RIV CB - Analytical Chemistry, Separation R&D Projects GAP301/11/2055 GA ČR - Czech Science Foundation (CSF) Institutional support UIACH-O - RVO:68081715 UT WOS 000326684000016 DOI 10.1016/j.aca.2013.01.046 Annotation A dry film resist (DFR) chip compatible with the Agilent Bioanalyzer 2100 was designed and fabricated for use in the analysis of lactate in serum by chip isotachophoresis (ITP). The Agilent Bioanalyzer 2100 is a commercially available field deployable analytical instrument originally developed for the electrophoretic analysis of DNA, RNA and proteins. The DFR chip was designed for the ITP separation of lactate in human serum within 1 min and was made compatible with the Bioanalyzer after packaging in the plastic caddies normally used for the DNA chips. A 20-fold improvement in sensitivity was obtained for the DFR chips in comparison with the standard chips used in earlier work. The limit of detection and limit of quantification for lactate were 24 M and 80 M, respectively. This new approach enables the use of commercial platforms like the Agilent Bioanalyzer for new applications including the analysis of small molecules. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2014
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