Selected phenotypic features of BR91, a unique spirochaetal strainisolated from the Culex pipiens mosquito

0398744 - ÚBO 2015 RIV DE eng J - Journal Article
Šikutová, Silvie - Buňková, L. - Krejčí, E. - Halouzka, Jiří - Sanogo, Y. O. - Rudolf, Ivo
Selected phenotypic features of BR91, a unique spirochaetal strainisolated from the Culex pipiens mosquito.
Microbiological Research. Roč. 169, 5-6 (2014), s. 348-352. ISSN 0944-5013. E-ISSN 1618-0623
R&D Projects: GA ČR GA206/00/1234; GA ČR GA206/03/0726
Institutional support: RVO:68081766
Keywords : Spirochaete * Culex pipiens * Enzyme activity * SDS-PAGE * FAMEa
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.561, year: 2014

Growth temperature range, resistance to selective antibiotics, activities of 23 enzymes, protein fin-gerprints and fatty acids composition of the spirochaetal strain BR91, isolated from the Culex pipiensmosquito, were tested. The spirochaetes were grown in BSK-H Complete liquid medium. The opti-mal in vitro growth temperature of the strain was 33◦C. Strain BR91 was sensitive to trimethoprim,nalidixic acid, 5-fluorouracil, and tolerated phosphomycin. The strain produced acid and alkaline phos-phatase, esterase (C4), esterase–lipase (C8), leucine arylamidase, naphthol-AS-BI-phosphohydrolase and -fucosidase. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) assay revealed sev-eral major proteins in the size range of 13–16 kDa, 22–30 kDa and 37–131 kDa. Fatty acid methyl ester(FAME) analysis showed that C14:0, C16:0, C18:1 9c and summed feature 5 (C18:2 6,9c and/or C18:0anteiso)are major fatty acids. This study highlights certain phenotypic differences between strain BR91 and theLyme disease spirochaete Borrelia burgdorferi, and supports the hypothesis that strain BR91 represents aunique taxonomical entity in a system of spirochaetal species.
Permanent Link: http://hdl.handle.net/11104/0226211