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Avidin-conjugated polymers with monobiotinylated antibody fragments: a new strategy for the noncovalent attachment of recombinant proteins for polymer therapeutics
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SYSNO ASEP 0393213 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Avidin-conjugated polymers with monobiotinylated antibody fragments: a new strategy for the noncovalent attachment of recombinant proteins for polymer therapeutics Author(s) Laga, Richard (UMCH-V) RID, ORCID
Pola, Robert (UMCH-V) RID, ORCID
Ulbrich, Karel (UMCH-V) RID
Hořejší, Magdalena (UMG-J)
Sieglová, Irena (UMG-J)
Král, Vlastimil (UMG-J) RID
Fábry, Milan (UMG-J) RID
Pechar, Michal (UMCH-V) RID, ORCIDSource Title Journal of Bioactive and Compatible Polymers. - : Sage - ISSN 0883-9115
Roč. 28, č. 3 (2013), s. 289-299Number of pages 11 s. Language eng - English Country GB - United Kingdom Keywords avidin-biotin complex ; polymer therapeutics ; drug targeting Subject RIV CD - Macromolecular Chemistry R&D Projects GCP207/12/J030 GA ČR - Czech Science Foundation (CSF) IAAX00500803 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) Institutional support UMCH-V - RVO:61389013 ; UMG-J - RVO:68378050 CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000323667500008 DOI 10.1177/0883911513486225 Annotation The high affinity and specificity between avidin and biotin were employed to bind a recombinant single-chain antibody fragment to synthetic hydrophilic polymer drug carriers. Two semitelechelic polymers, based on poly(ethylene glycol) and poly[N-(2-hydroxypropyl)methacrylamide], each containing a single thiol end group, were conjugated to dithiopyridyl-modified avidin. The biotinylated recombinant single-chain antibody fragment of the M75 antibody was then noncovalently bound to the polymer-avidin conjugates. The recombinant protein was chosen as a targeting ligand against carbonic anhydrase IX, a marker overexpressed by tumor cells of various human carcinomas. The antigen-binding affinity of the polymer–single-chain antibody fragment complex was confirmed by enzyme-linked immuno sorbent assay (ELISA). This approach provides an original, nondestructive way of preparing supramolecular systems intended for targeted delivery of therapeutics utilizing modern chemical procedures, including reversible addition–fragmentation chain-transfer polymerization and recombinant DNA techniques. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2014
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