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Raman spectroscopy for bacterial identification and characterization
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SYSNO ASEP 0388104 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Raman spectroscopy for bacterial identification and characterization Author(s) Bernatová, Silvie (UPT-D) RID, SAI
Samek, Ota (UPT-D) RID, ORCID, SAI
Pilát, Zdeněk (UPT-D) RID, SAI, ORCID
Šerý, Mojmír (UPT-D) RID, SAI
Ježek, Jan (UPT-D) RID, ORCID, SAI
Krzyžánek, Vladislav (UPT-D) RID, ORCID, SAI
Zemánek, Pavel (UPT-D) RID, SAI, ORCID
Růžička, F. (CZ)Number of authors 8 Source Title CPS 2012. 18th Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics (Proceedings of SPIE Vol.8697). - Bellingham : SPIE, 2012 - ISBN 978-0-8194-9481-8 Pages 86970y: 1-6 Number of pages 6 s. Publication form Print - P Action CPS 2012. Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics /18./ Event date 03.09.2012-07.09.2012 VEvent location Ostravice Country CZ - Czech Republic Event type EUR Language eng - English Country US - United States Keywords Raman spectroscopy ; Raman tweezers ; bacteria ; yeast biofilm ; PCA Subject RIV BH - Optics, Masers, Lasers R&D Projects ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GAP205/11/1687 GA ČR - Czech Science Foundation (CSF) EE2.4.31.0016 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UPT-D - RVO:68081731 UT WOS 000319864100034 EID SCOPUS 84875933565 DOI 10.1117/12.2010539 Annotation The main goal of our investigation is to use Raman tweezers technique so that the responce of Raman scattering on microorganisms suspended in liquid media (bacteria, algae and yeast cells in microfluidic chips) can be used to identify different species. The investigations presented here include identification of different bacteria strains (biofilm-positive and biofilm-negative) and yeast cells by using principal component analysis (PCA). The main driving force behind our investigation was a common problem in the clinical microbiology laboratory - how to distinguish between contaminant and invasive isolates. Invasive bacterial/yeast isolates can be assumed to form a biofilm, while isolates which do not form a biofilm can be treated as contaminant. Thus, the latter do not represent an important virulence factor. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2013
Number of the records: 1