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Caspase-7 in molar tooth development
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SYSNO ASEP 0386731 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Caspase-7 in molar tooth development Author(s) Matalová, Eva (UZFG-Y) ORCID
Vanden Berghe, T. (BE)
Švandová, Eva (UZFG-Y) RID, ORCID
Vandenabeele, P. (BE)
Healy, C. (GB)
Sharpe, P. T. (GB)
Tucker, A. S. (GB)Source Title Archives of Oral Biology. - : Elsevier - ISSN 0003-9969
Roč. 57, č. 11 (2012), s. 1474-1481Number of pages 8 s. Language eng - English Country US - United States Keywords tooth ; apoptosis ; mineralisation Subject RIV FF - HEENT, Dentistry R&D Projects IAA600450904 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000312047200004 DOI https://doi.org/10.1016/j.archoralbio.2012.06.009 Annotation Objectives: The primary enamel knot (PEK) is a population of cells that shows spatio-temporal restricted apoptosis during tooth development. It has been shown that caspase-9 and Apaf-1 are essential for apoptosis in the PEK as well as the central caspase-3. Caspase-7, as another executioner member in the caspase machinery, is considered to have caspase-3 like properties. Design: The aim of this study was to detect caspase-7 activation during molar tooth development with a special focus on the cells of the PEK and to correlate the expression with the pattern of apoptosis and caspase-3 activation. Apoptosis in the PEK was investigated in caspase-7 deficient mice to examine the functional consequence of loss of this specific caspase. In addition, odontoblasts and ameloblasts, which are known to undergo cell death during their secretory and maturation stages, were investigated. Results: Cleaved caspase-7 was found in the apoptotic region of the PEK, however, caspase-7-deficient mice still possessed apoptotic cells in the PEK in a similar distribution to the wild type. Caspase-7 is therefore not essential for apoptosis in the PEK. Notably, cleaved caspase-7-positive cells were found at later stages in odontoblasts and ameloblasts, but expression did not correlate with apoptosis in these tissues. Conclusions: The results indicate a non-essential apoptotic role of caspase-7 in the PEK apoptosis but suggest also possible non-apoptotic functions for caspase-7 in tooth development. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2013
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