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Simple protein structure-sensitive chronopotentiometric analysis with dithiothreitol-modified Hg electrodes
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SYSNO ASEP 0383508 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Simple protein structure-sensitive chronopotentiometric analysis with dithiothreitol-modified Hg electrodes Author(s) Ostatná, Veronika (BFU-R) RID, ORCID
Černocká, Hana (BFU-R) RID, ORCID
Paleček, Emil (BFU-R) RID, ORCIDNumber of authors 3 Source Title Bioelectrochemistry. - : Elsevier - ISSN 1567-5394
Roč. 87, SI (2012), s. 84-88Number of pages 5 s. Language eng - English Country NL - Netherlands Keywords protein electroanalysis ; DTT-modified electrodes ; electrocatalysis Subject RIV BO - Biophysics R&D Projects KJB100040901 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) GAP301/11/2055 GA ČR - Czech Science Foundation (CSF) LC06035 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50040507 - BFU-R (2005-2011) AV0Z50040702 - BFU-R (2007-2013) UT WOS 000309033000014 DOI 10.1016/j.bioelechem.2012.01.004 Annotation In the paper the authors studied the properties of the dithiothreitol (DTT) layer at the hanging mercury drop electrode. At higher DTT concentrations, a densely packed pinhole-free layer is formed with the DTT molecules bound to the electrode surface by a single -SH group, oriented perpendicularly to the surface. If a sufficiently high DTT concentration is used, proteins can be co-adsorbed with DTT on liquid Hg or solid amalgam electrodes without the loss of sensitivity for changes in protein structures. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2013
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