Capillary electrophoretic profiling of tryptic digests of water soluble proteins from Bacillus thuringiensis-transgenic and non-transgenic maize species

0378012 - ÚOCHB 2013 RIV NL eng J - Journal Article
Sázelová, Petra - Kašička, Václav - Leon, C. - Ibanez, E. - Cifuentes, A.
Capillary electrophoretic profiling of tryptic digests of water soluble proteins from Bacillus thuringiensis-transgenic and non-transgenic maize species.
Food Chemistry. Roč. 134, č. 3 (2012), s. 1607-1615. ISSN 0308-8146. E-ISSN 1873-7072
R&D Projects: GA ČR(CZ) GA203/08/1428
Grant - others:AV ČR(CZ) 2008CZ0019
Institutional research plan: CEZ:AV0Z40550506
Keywords : Bt-transgenic maize * capillary zone electrophoresis * maize proteins
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 3.334, year: 2012

Capillary zone electrophoresis has been applied to separation and characterization of enzymatic (tryptic) hydrolyzates of water-soluble proteins from Bacillus thuringiensis (Bt)-transgenic (Aristis-Bt) and two native non-transgenic (Aristis and Coventry) maize varieties. Some significant relative qualitative and quantitative differences in CZE-UV profiling of tryptic protein digests were found, which can be potentially used to differentiate transgenic Aristis Bt and non-transgenic Aristis varieties or two native non-transgenic varieties, Aristis and Coventry.
Permanent Link: http://hdl.handle.net/11104/0210004