Global gene expression changes in human urothelial cells exposed to low-level monomethylarsonous acid

0377194 - BC 2013 RIV IE eng J - Journal Article
Medeiros, M. - Zheng, X. - Novák, Petr - Wnek, S.M. - Chyan, V. - Escudero-Lourdes, C. - Gandolfi, A.J.
Global gene expression changes in human urothelial cells exposed to low-level monomethylarsonous acid.
Toxicology. Roč. 291, 1-3 (2012), s. 102-112. ISSN 0300-483X
Institutional research plan: CEZ:AV0Z50510513
Institutional support: RVO:60077344
Keywords : HUMAN BLADDER CELLS * METHYLATED TRIVALENT ARSENICALS * MALIGNANT-TRANSFORMATION0300
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 4.017, year: 2012

Bladder cancer has been associated with chronic arsenic exposure. Monomethylarsonous acid [MMA(III)] is a metabolite of inorganic arsenic and has been shown to transform an immortalized urothelial cell line (UROtsa). MMA(III) was used as a model arsenical to examine the mechanisms of arsenical-induced transformation of urothelium. A microarray analysis was performed to assess the transcriptional changes in UROtsa. The analysis revealed only minor changes in gene expression at 1 and 2 months of exposure, contrasting with substantial changes observed at 3 months of exposure. The gene expression changes at 3 months were analyzed showing distinct alterations in biological processes and pathways such as a response to oxidative stress, enhanced cell proliferation, anti-apoptosis, MAPK signaling, as well as inflammation.
Permanent Link: http://hdl.handle.net/11104/0209423