Number of the records: 1
Antibody repertoire development in fetal and neonatal piglets. XX. B cell lymphogenesis is absent in the ileal Peyer´s patches, their repertoire development is antigen dependent, and they are not required for B cell maintenance
- 1.
SYSNO ASEP 0369862 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Antibody repertoire development in fetal and neonatal piglets. XX. B cell lymphogenesis is absent in the ileal Peyer´s patches, their repertoire development is antigen dependent, and they are not required for B cell maintenance Author(s) Butler, J. (US)
Santiago-Mateo, K. (US)
Sun, X.-Z. (US)
Wertz, N. (US)
Šinkora, Marek (MBU-M) RID, ORCID
Francis, D. (US)Source Title Journal of Immunology. - : American Association of Immunologists - ISSN 0022-1767
Roč. 187, č. 10 (2011), s. 5141-5149Number of pages 9 s. Language eng - English Country US - United States Keywords neonatal piglets ; Peyer’s patches ; b cell Subject RIV EC - Immunology R&D Projects ME09089 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000296767300023 DOI 10.4049/jimmunol.1101871 Annotation The continuous ileal Peyer’s patches (IPP) of sheep are regarded as a type of mammalian bursal equivalent where B cells diversify their repertoire in an Ag-independent fashion. Anatomically and developmentally similar IPP occur in swine. Resection of ~90% of the IPP in piglets at birth did not alter Ig levels in serum and secretions or retard diversification of the Ab repertoire when animals were maintained in isolators and colonized with a defined gut flora. Resection or sham surgery elevated IgG and IgA in serum and in lavage fluid from the gut, lung, and in saliva. No changes in the frequency of IgG-, IgA-, and IgM-containing cells in the spleen and peripheral lymph node were observed. Using an index that quantifies diversification of the VDJ repertoire, no differences were seen in three secondary lymphoid tissues between piglets lacking IPP and colonized controls, whereas both groups displayed >10-fold greater diversification than did late-term fetal piglets or piglets maintained germ-free. Somatic hypermutation was very low in fetal IPP and the IPP of germ-free piglets but increased 3- to 5-fold after colonization. D–J signal joint circles were not recovered in IPP, and V–DJ signal joint circles were 5-fold lower than in bone marrow and similar to those in thymus and spleen Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2012
Number of the records: 1