Number of the records: 1  

Histone deacetylase activity modulates alternative splicing

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    SYSNO ASEP0364989
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleHistone deacetylase activity modulates alternative splicing
    Author(s) Hnilicová, Jarmila (UMG-J)
    Hozeifi, Samira (UMG-J)
    Dušková, Eva (UMG-J)
    Icha, Jaroslav (UMG-J)
    Tománková, Tereza (UMG-J)
    Staněk, David (UMG-J) RID
    Source TitlePLoS ONE. - : Public Library of Science - ISSN 1932-6203
    Roč. 6, č. 2 (2011), e16727
    Number of pages11 s.
    Languageeng - English
    CountryUS - United States
    Keywordshistone deacetylase ; alternative splicing ; chromatin
    Subject RIVEB - Genetics ; Molecular Biology
    R&D ProjectsGAP305/10/0424 GA ČR - Czech Science Foundation (CSF)
    KAN200520801 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
    CEZAV0Z50520514 - UMG-J (2005-2011)
    UT WOS000287036200036
    DOI10.1371/journal.pone.0016727
    AnnotationIncreasing evidence suggests that splicing decisions are largely made when the nascent RNA is still associated with chromatin. Here we demonstrate that activity of histone deacetylases (HDACs) influences splice site selection. Using splicing-sensitive microarrays, we identified ca 700 genes whose splicing was altered after HDAC inhibition. We provided evidence that HDAC inhibition induced histone H4 acetylation and increased RNA Polymerase II (Pol II) processivity along an alternatively spliced element. In addition, HDAC inhibition reduced co-transcriptional association of the splicing regulator SRp40 with the target fibronectin exon. We further showed that the depletion of HDAC1 had similar effect on fibronectin alternative splicing as global HDAC inhibition. Importantly, this effect was reversed upon expression of mouse HDAC1 but not a catalytically inactive mutant. These results provide a molecular insight into a complex modulation of splicing by HDACs and chromatin modifications.
    WorkplaceInstitute of Molecular Genetics
    ContactNikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
    Year of Publishing2012
Number of the records: 1  

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