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Primer Sequence Disclosure: A Clarification of the MIQE Guidelines
- 1.0364331 - BTÚ 2012 RIV US eng J - Journal Article
Bustin, S.A. - Benes, V. - Garson, J.A. - Hellemans, J. - Huggett, J. - Kubista, Mikael - Mueller, R. - Nolan, T. - Pfaffl, M.W. - Shipley, G.L. - Vandesompele, J. - Wittwer, C.T.
Primer Sequence Disclosure: A Clarification of the MIQE Guidelines.
Clinical Chemistry. Roč. 57, č. 6 (2011), s. 919-921. ISSN 0009-9147. E-ISSN 1530-8561
Institutional research plan: CEZ:AV0Z50520701
Keywords : real-time PCR * MIQE * quality
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 7.905, year: 2011
The MIQE guidelines has rapidly become standard reporting procedure when writing up and presenting real-time quantitative pCR (qPCR) data. Accoeding to the originál requirement of MIQE full disclosure of primer sequence information is requested. This requirement is not possible to meet when using commersial primers from some leading vendors, since they disclose to clients only limited amount of informatik about the primers they supply. Recently they increased the information provided to clients to make experiments easier to design and easier to reproduce. In this letter the MIQE guidelines are amended to allow also these primers to be used in MIQE complient reports and publications.
Permanent Link: http://hdl.handle.net/11104/0199843
Number of the records: 1