A new approach to the isolation and characterization of wheat flour allergens

Šotkovský, Petr; Sklenář, Jan; Halada, Petr; Cinová, Jana; Šetinová, I.; Kainarová, Andrea; Goliáš, Jaroslav; Pavlásková, Kateřina; Honzová, S.; Tučková, Ludmila

doi:https://dx.doi.org/10.1111/j.1365-2222.2011.03766.x

Number of the records: 1

A new approach to the isolation and characterization of wheat flour allergens

1.

SYSNO ASEP	0362802
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	A new approach to the isolation and characterization of wheat flour allergens
Author(s)	Šotkovský, Petr (MBU-M) Sklenář, Jan (MBU-M) Halada, Petr (MBU-M)_{RID, ORCID} Cinová, Jana (MBU-M) Šetinová, I. (CZ) Kainarová, Andrea (MBU-M) Goliáš, Jaroslav (MBU-M)_RID Pavlásková, Kateřina (MBU-M) Honzová, S. (CZ) Tučková, Ludmila (MBU-M)_RID
Source Title	Clinical and Experimental Allergy - ISSN 0954-7894 Roč. 41, č. 7 (2011), s. 1031-1043
Number of pages	13 s.
Language	eng - English
Country	US - United States
Keywords	basophil activation ; IgE antibodies ; isolation
Subject RIV	EC - Immunology
R&D Projects	GA310/07/0414 GA ČR - Czech Science Foundation (CSF)
	GD310/08/H077 GA ČR - Czech Science Foundation (CSF)
	IAA500200801 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	2B06155 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LC07017 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50200510 - MBU-M (2005-2011)
UT WOS	000292699700016
DOI	10.1111/j.1365-2222.2011.03766.x
Annotation	The incidence of food allergy to wheat is increasing. Its diagnosis depends on the purity of major allergens and their inclusion in tests. Isolation and characterization of wheat allergens are therefore of utmost importance. Objective To purify and identify wheat flour allergens most frequently recognized by patients IgE antibodies and to study their allergenicity. Methods Water/salt-soluble extracts from wheat flour were prepared and separated using a combination of ultrafiltration, isoelectric focusing and liquid chromatography. Purified proteins were analysed by immunoblotting using pooled sera from patients with atopic dermatitis who possessed IgE specific to wheat.
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2012

Number of the records: 1