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Effect of epidermal growth factor-like peptides on pig cumulus cell expansion, oocyte maturation and acquisition of developmental competence in vitro: comparison with gonadotropins
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SYSNO ASEP 0359775 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Effect of epidermal growth factor-like peptides on pig cumulus cell expansion, oocyte maturation and acquisition of developmental competence in vitro: comparison with gonadotropins Author(s) Procházka, Radek (UZFG-Y) RID, ORCID
Petlach, Michal (UZFG-Y)
Nagyová, Eva (UZFG-Y) RID, ORCID
Němcová, Lucie (UZFG-Y) RIDSource Title Reproduction. - : BioScientifica - ISSN 1470-1626
Roč. 141, č. 4 (2011), s. 425-435Number of pages 11 s. Language eng - English Country GB - United Kingdom Keywords activatd protein kinase ; EGF like factor ; meiotic resumption Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA523/08/0111 GA ČR - Czech Science Foundation (CSF) QI101A166 GA MZe - Ministry of Agriculture (MZe) CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000288500500005 DOI 10.1530/REP-10-0418 Annotation The aim of this work was to assess the FSH-stimulated expression of epidermal growth factor (EGF)-like peptides in cultured cumulus-oocyte complexes (COCs) and to find out the effect of the peptides on cumulus expansion, oocyte maturation and acquisition of developmental competence in vitro. FSH promptly stimulated expression of amphiregulin (AREG) and epiregulin (EREG), but notbetacellulin (BTC) in the cultured COCs. Both FSH and the EGF-like peptides significantly stimulated expression of expansion related genes (PTGS2, TNFAIP6 and HAS2) in the COCs and stimulated maturation of oocytes and expansion of cumulus cells. In contrast to FSH, AREG and EREG neither stimulated expression of CYP11A1 in the COCs nor an increase in progesterone production by cumulus cells. Significantly more oocytes stimulated with AREG and/or EREG developed to blastocyst stage after parthenogenetic activation when compared to oocytes stimulated with FSH alone or combinations of FSH/LH or PMSG/hCG. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2012
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