Number of the records: 1  

Development of a Multilocus Sequence Tool for Typing Cryptosporidium muris and Cryptosporidium andersoni

  1. 1.
    SYSNO ASEP0358458
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleDevelopment of a Multilocus Sequence Tool for Typing Cryptosporidium muris and Cryptosporidium andersoni
    Author(s) Feng, Y. (CN)
    Yang, W. (US)
    Ryan, U. M. (AU)
    Zhang, L. (CN)
    Kváč, Martin (BC-A) RID, RID, ORCID
    Koudela, Břetislav (BC-A) ORCID
    Modrý, David (BC-A) RID, ORCID
    Li, N. (CN)
    Fayer, R. (US)
    Xiao, L. (US)
    Source TitleJournal of Clinical Microbiology - ISSN 0095-1137
    Roč. 49, č. 1 (2011), s. 34-41
    Number of pages8 s.
    Languageeng - English
    CountryUS - United States
    KeywordsASTERN UNITED-STATES ; RIBOSOMAL-RNA GENE ; PHYLOGENETIC ANALYSIS ; MOLECULAR ANALYSIS ; NATURAL INFECTION ; DAIRY-CATTLE ; PREVALENCE ; GENOTYPES ; HUMANS ; KENYA
    Subject RIVGJ - Animal Vermins ; Diseases, Veterinary Medicine
    CEZAV0Z60220518 - PAU-O, BC-A (2005-2011)
    UT WOS000285787100003
    DOI10.1128/JCM.01329-10
    AnnotationAlthough widely used for the characterization of the transmission of intestinal Cryptosporidium spp., genotyping tools are not available for C. muris and C. andersoni, 2 of the most common gastric spp. infecting mammals. In this study, we screened the C. muris whole-genome sequencing data - among the 13 potential loci (6 microsatellite and 7 minisatellite loci) evaluated by PCR and DNA sequencing, 4 were chosen. DNA sequence analyses of 44 samples showed the presence of 5 to 10 subtypes of C. muris and 1 to 4 subtypes of C. andersoni at each locus. Altogether, 11 C. muris and 7 C. andersoni multilocus sequence typing (MLST) subtypes were detected among the 16 C. muris and 12 C. andersoni specimens successfully sequenced at all 4 loci. In all analyses, the C. muris isolate (TS03) that originated from an East African mole rat differed significantly from other C. muris isolates, approaching the extent of genetic differences between C. muris and C. andersoni.
    WorkplaceBiology Centre (since 2006)
    ContactDana Hypšová, eje@eje.cz, Tel.: 387 775 214
    Year of Publishing2012
Number of the records: 1  

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