Number of the records: 1
Development of a Multilocus Sequence Tool for Typing Cryptosporidium muris and Cryptosporidium andersoni
- 1.
SYSNO ASEP 0358458 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Development of a Multilocus Sequence Tool for Typing Cryptosporidium muris and Cryptosporidium andersoni Author(s) Feng, Y. (CN)
Yang, W. (US)
Ryan, U. M. (AU)
Zhang, L. (CN)
Kváč, Martin (BC-A) RID, RID, ORCID
Koudela, Břetislav (BC-A) ORCID
Modrý, David (BC-A) RID, ORCID
Li, N. (CN)
Fayer, R. (US)
Xiao, L. (US)Source Title Journal of Clinical Microbiology - ISSN 0095-1137
Roč. 49, č. 1 (2011), s. 34-41Number of pages 8 s. Language eng - English Country US - United States Keywords ASTERN UNITED-STATES ; RIBOSOMAL-RNA GENE ; PHYLOGENETIC ANALYSIS ; MOLECULAR ANALYSIS ; NATURAL INFECTION ; DAIRY-CATTLE ; PREVALENCE ; GENOTYPES ; HUMANS ; KENYA Subject RIV GJ - Animal Vermins ; Diseases, Veterinary Medicine CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000285787100003 DOI 10.1128/JCM.01329-10 Annotation Although widely used for the characterization of the transmission of intestinal Cryptosporidium spp., genotyping tools are not available for C. muris and C. andersoni, 2 of the most common gastric spp. infecting mammals. In this study, we screened the C. muris whole-genome sequencing data - among the 13 potential loci (6 microsatellite and 7 minisatellite loci) evaluated by PCR and DNA sequencing, 4 were chosen. DNA sequence analyses of 44 samples showed the presence of 5 to 10 subtypes of C. muris and 1 to 4 subtypes of C. andersoni at each locus. Altogether, 11 C. muris and 7 C. andersoni multilocus sequence typing (MLST) subtypes were detected among the 16 C. muris and 12 C. andersoni specimens successfully sequenced at all 4 loci. In all analyses, the C. muris isolate (TS03) that originated from an East African mole rat differed significantly from other C. muris isolates, approaching the extent of genetic differences between C. muris and C. andersoni. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2012
Number of the records: 1