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New model system for testing effects of flavonoids on doxorubicin-related formation of hydroxyl radicals
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SYSNO ASEP 0358366 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title New model system for testing effects of flavonoids on doxorubicin-related formation of hydroxyl radicals Author(s) Souček, P. (CZ)
Kondrová, E. (CZ)
Heřmánek, J. (CZ)
Stopka, Pavel (UACH-T) SAI
Boumendjel, A. (FR)
Ueng, YF. (TW)
Gut, I. (CZ)Source Title Anti-cancer Drugs - ISSN 0959-4973
Roč. 22, č. 2 (2011), s. 176-184Number of pages 9 s. Language eng - English Country US - United States Keywords doxorubicin ; electron spin resonance ; flavonoids hydroxyl radicals Subject RIV FD - Oncology ; Hematology CEZ AV0Z40320502 - UACH-T (2005-2011) UT WOS 000285545200007 DOI 10.1097/CAD.0b013e328341a17b Annotation Doxorubicin belongs to anthracycline cytotoxic drugs and it is widely used as a major therapeutic agent in the treatment of various types of tumors. However, its therapeutic use is limited by the development of myelosuppression and cardiotoxicity after a specific cumulative dose is reached. The aim of this study was to investigate the effect of flavonoids, either natural or synthetic on doxorubicin-mediated formation of oxidative stress implicated in doxorubicin toxicity. Doxorubicin caused a concentration-dependent increase in the formation of hydroxyl radicals in minipig liver microsomes used as an in-vitro model system. When bacterial membranes heterologously expressing human NADPH cytochrome-P450 oxidoreductase were incubated with doxorubicin, formation of the superoxide radical under aerobic conditions and the doxorubicin-semiquinone radical under anaerobic conditions was detected. Workplace Institute of Inorganic Chemistry Contact Jana Kroneislová, krone@iic.cas.cz, Tel.: 311 236 931 Year of Publishing 2012
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