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Assessing oestrogenic effects of brominated flame retardants Hexabromocyclododecane and Tetrabromobisphenol A on MCF-7 cells
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SYSNO ASEP 0356209 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Assessing oestrogenic effects of brominated flame retardants Hexabromocyclododecane and Tetrabromobisphenol A on MCF-7 cells Author(s) Dorosh, Andriy (BTO-N) RID
Děd, Lukáš (BTO-N) RID
Elzeinová, Fatima (BTO-N) RID
Pěknicová, Jana (BTO-N) RIDNumber of authors 4 Source Title Folia Biologica. - : Univerzita Karlova v Praze - ISSN 0015-5500
Roč. 56, - (2010), s. 35-39Number of pages 5 s. Language eng - English Country CZ - Czech Republic Keywords endocrine disruptors ; BRF - brominated flame retardant ; MCF-7 cells ; TFF1 - trefoil factor Subject RIV DN - Health Impact of the Environment Quality R&D Projects 1M06011 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) 2B06151 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50520701 - BTO-N (2007-2013) UT WOS 000288623700007 Annotation We used the proliferation test (E-screen assay) in MCF-7 breast cancer cells and reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis of TFF1 gene expression to analyze estrogenicity of the studied compounds. RT-qPCR has proven to be a fast and valuable molecular technique in gene expression quantification. HBCD but not TBBPA increased cell proliferation in MCF-7 cells and up-regulates TFF1 gene expression in a concentration-dependent manner. Anti-estrogen ICI 182,780 inhibits up-regulation of TFF1 by HBCD. We showed that HBCD displays estrogen-like effects on MCF-7 cells. TBBPA, on the other hand, has not shown any estrogenic effect mediated by the estrogen receptor alpha. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2012
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