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Senescence-associated heterochromatin foci are dispensable for cellular senescence, occur in a cell type- and insult-dependent manner, and follow expression of p16 (ink4a)
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SYSNO ASEP 0356108 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Senescence-associated heterochromatin foci are dispensable for cellular senescence, occur in a cell type- and insult-dependent manner, and follow expression of p16 (ink4a) Author(s) Košař, Martin (UMG-J)
Bartkova, J. (DK)
Hubáčková, Soňa (UMG-J) RID
Hodný, Zdeněk (UMG-J) RID
Lukas, J. (DK)
Bártek, Jiří (UMG-J) RIDSource Title Cell Cycle. - : Taylor & Francis - ISSN 1538-4101
Roč. 10, č. 3 (2011), s. 457-468Number of pages 12 s. Language eng - English Country US - United States Keywords genotoxic and replicative stress ; senescence-associated heterochromatin foci ; DNA damage response Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA204/08/1418 GA ČR - Czech Science Foundation (CSF) GA301/08/0353 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000286827100023 DOI 10.4161/cc.10.3.14707 Annotation Primary human fibroblasts undergoing oncogene-induced or replicative senescence are known to form senescence-associated heterochromatin foci (SAHF), nuclear DNA domains stained densely by DAPI and enriched for histone modifications including lysine9-trimethylated histone H3. While cellular senescence occurs also in premalignant human lesions, it is unclear how universal SAHF formation is among various cell types, under diverse stresses and whether SAHF occur in vivo. Here, we report that human primary fibroblasts and primary keratinocytes undergoing replicative senescence or premature senescence induced by oncogenic H-Ras, diverse chemotherapeutics and bacterial cytolethal distending toxin, show differential capacity to form SAHF indicating that unlike the widely present DNA damage response marker γH2AX, SAHF is not a common feature of cellular senescence. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2011
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