Number of the records: 1
Structure of the human FOXO4-DBD-DNA complex at 1.9 A resolution reveals new details of FOXO binding to the DNA
- 1.
SYSNO ASEP 0354847 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Structure of the human FOXO4-DBD-DNA complex at 1.9 A resolution reveals new details of FOXO binding to the DNA Author(s) Bouřa, E. (CZ)
Řežábková, L. (CZ)
Brynda, Jiří (UMG-J) RID
Obšilová, Veronika (FGU-C) RID, ORCID, SAI
Obšil, T. (CZ)Source Title Acta Crystallographica Section D-Biological Crystallography. - : WILEY-BLACKWELL - ISSN 0907-4449
Roč. 66, č. 12 (2010), s. 1351-1357Number of pages 7 s. Language eng - English Country DK - Denmark Keywords transcription factor AFX ; posttranslational modification ; specificity Subject RIV EB - Genetics ; Molecular Biology R&D Projects IAA501110801 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) LC554 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50520514 - UMG-J (2005-2011) AV0Z50110509 - FGU-C (2005-2011) UT WOS 000284881300012 DOI 10.1107/S0907444910042228 Annotation Here, the 1.9 A resolution crystal structure of the DNA-binding domain of human FOXO4 (FOXO4-DBD) bound to a 13 bp DNA duplex containing a FOXO consensus binding sequence is reported. The structure shows a similar recognition of the core sequence as has been shown for two other FOXO proteins. Helix H3 is docked into the major groove and provides all of the base-specific contacts, while the N-terminus and wing W1 make additional contacts with the phosphate groups of DNA. In contrast to other FOXO-DBD-DNA structures, the loop between helices H2 and H3 has a different conformation and participates in DNA binding. In addition, the structure of the FOXO4-DBD-DNA complex suggests that both direct water-DNA base contacts and the unique water-DNA base contacts and the unique water-network interactions contribute to FOXO-DBD binding to the DNA in a sequence-specific manner. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2011
Number of the records: 1