The expression of NADPH oxidases and production of reactive oxygen species by human lung adenocarcinoma epithelial cell line A549

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SYSNO ASEP	0353772
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	The expression of NADPH oxidases and production of reactive oxygen species by human lung adenocarcinoma epithelial cell line A549
Author(s)	Kolářová, Hana (BFU-R)_RID Binó, Lucia (BFU-R)_ORCID Pejchalová, Kateřina (BFU-R) Kubala, Lukáš (BFU-R)_{RID, ORCID}
Number of authors	4
Source Title	Folia Biologica. - : Univerzita Karlova v Praze - ISSN 0015-5500 Roč. 56, č. 5 (2010), s. 211-217
Number of pages	7 s.
Language	eng - English
Country	CZ - Czech Republic
Keywords	lung epithelial cells ; reactive oxygen species ; NADPH oxidase
Subject RIV	BO - Biophysics
R&D Projects	GA524/06/1197 GA ČR - Czech Science Foundation (CSF)
	GA524/08/1753 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50040507 - BFU-R (2005-2011)
	AV0Z50040702 - BFU-R (2007-2013)
UT WOS	000284254900004
Annotation	Human lung epithelial cell line A549 was found to significantly express NOX1, NOX2, DUOX1 and DUOX2 and ROS production was detected with fluorometric probes 2,7-dichlorofluorescein- diacetate, dihydroethidium, and amplex red or colorimetric probe nitrobluetetrazolium. These results revealed differences among methods used for ROS formation measurement by cell line A549 and highlighted the sensitivity of fluorometric determination for this purpose.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2011

Number of the records: 1