Current Protocols in Chemical Biology

Number of the records: 1

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SYSNO ASEP	0353703
Document Type	M - Monograph Chapter
R&D Document Type	Monograph Chapter
Title	Cross-coupling Modification of Nucleoside Triphosphates, PEX, and PCR Construction of Base-Modified DNA
Author(s)	Macíčková-Cahová, Hana (UOCHB-X)_{RID, ORCID} Vrábel, Milan (UOCHB-X)_{RID, ORCID} Hocek, Michal (UOCHB-X)_{RID, ORCID}
Number of authors	3
Source Title	Current Protocols in Chemical Biology, 2. - New York : Wiley Interscience, 2010 - ISBN 9780470559277
Pages	s. 1-14
Number of pages	14 s.
Number of pages	550
Language	eng - English
Country	US - United States
Keywords	nucleoside triphosphates ; DNA polymerase ; cross-coupling ; DNA conjugates
Subject RIV	CC - Organic Chemistry
CEZ	AV0Z40550506 - UOCHB-X (2005-2011)
DOI	10.1002/9780470559277.ch090177
Annotation	A novel, efficient, two-step methodology is presented for construction of base-modified oligonucleotides or DNA involving aqueous cross-coupling reactions of halogenated nucleoside triphosphates (dNTPs) with terminal acetylenes or arylboronic acids, followed by polymerase incorporation of the modified dNTPs either using primer extension (PEX) or polymerase chain reaction (PCR).
Workplace	Institute of Organic Chemistry and Biochemistry
Contact	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418
Year of Publishing	2011

Number of the records: 1