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Analysis and three-dimensional visualization of collagen in artificial scaffolds using nonlinear microscopy techniques
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SYSNO ASEP 0353572 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Analysis and three-dimensional visualization of collagen in artificial scaffolds using nonlinear microscopy techniques Author(s) Filová, Eva (UEM-P) RID, ORCID
Burdíková, Zuzana (FGU-C) RID
Rampichová, Michala (UEM-P) RID, ORCID
Bianchini, P. (IT)
Čapek, Martin (FGU-C) RID, ORCID
Košťáková, E. (CZ)
Amler, Evžen (UEM-P) RID
Kubínová, Lucie (FGU-C) RID, ORCIDSource Title Journal of Biomedical Optics - ISSN 1083-3668
Roč. 15, č. 6 (2010), 066011-1-066011-7Number of pages 7 s. Language eng - English Country US - United States Keywords biomedical materials ; biomedical optical imaging ; cellular biophysics Subject RIV BO - Biophysics R&D Projects LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GAP304/10/1307 GA ČR - Czech Science Foundation (CSF) IAA500390702 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z50390512 - UEM-P (2005-2011) AV0Z50390703 - UEM-P (2007-2013) AV0Z50110509 - FGU-C (2005-2011) UT WOS 000287171100044 DOI 10.1117/1.3509112 Annotation Extracellularly distributed collagen and chondrocytes seeded in gelatine and poly-ɛ-caprolactone scaffolds are visualized by two-photon excitation microscopy and second-harmonic generation . Joint application of TPEM and SHG imaging in combination with stereological measurements of collagen enables us not only to take high-resolution 3-D images, but also to quantitatively analyze the collagen volume and a spatial arrangement of cell-collagen-scaffold systems, which was previously impossible. This novel approach represents a powerful tool for the analysis of collagen-containing scaffolds with applications in cartilage tissue engineering. Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2011
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