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Porcine insulin receptor substrate 4 (IRS4) gene: cloning, polymorphism and association study
- 1.0353420 - ÚŽFG 2012 RIV NL eng J - Journal Article
Masopust, Martin - Vykoukalová, Z. - Knoll, Aleš - Bartenschlager, H. - Mileham, A. - Deeb, N. - Rohrer, G. A. - Čepica, Stanislav
Porcine insulin receptor substrate 4 (IRS4) gene: cloning, polymorphism and association study.
Molecular Biology Reports. Roč. 38, - (2010), 2611-2617. ISSN 0301-4851. E-ISSN 1573-4978
R&D Projects: GA ČR GA523/07/0353; GA ČR GAP502/10/1216
Institutional research plan: CEZ:AV0Z50450515
Keywords : PCR cloning * Polymorphism * IRS4
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.875, year: 2010
Using PCR and inverse PCR techniques we obtained a 4,498 bp nucleotide sequence FN424076 encompassing the complete coding sequence of the porcine insulin receptor substrate 4 (IRS4) gene and its proximal promoter. The 1,269 amino acid porcine protein deduced from the nucleotide sequence shares 92% identity with the human IRS4 and possesses the same domains and the same number of tyrosine phosphorylation motifs as the human protein. We detected substitution FN424076:g.96C<G in the promoter region that segregates in Meishan and a synonymous substitution FN424076:g.1829T<C in the coding sequence with allele C present only in Meishan.
Permanent Link: http://hdl.handle.net/11104/0192673
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Number of the records: 1