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Biolistic transformation of Schistosoma mansoni: Studies with modified reporter-gene constructs containing regulatory regions of protease genes
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SYSNO ASEP 0352015 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Biolistic transformation of Schistosoma mansoni: Studies with modified reporter-gene constructs containing regulatory regions of protease genes Author(s) Dvořák, Jan (BC-A)
Beckmann, S. (DE)
Lim, K.-C. (US)
Engel, J. C. (US)
Grevelding, C. G. (DE)
McKerrow, J. H. (US)
Caffrey, C. R. (US)Source Title Molecular and Biochemical Parasitology. - : Elsevier - ISSN 0166-6851
Roč. 170, č. 1 (2010), s. 37-40Number of pages 4 s. Language eng - English Country NL - Netherlands Keywords Schistosoma ; Protease ; Transgene ; Gene promoter ; Biolistics ; Electroporation Subject RIV EB - Genetics ; Molecular Biology CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000274915000005 DOI 10.1016/j.molbiopara.2009.11.001 Annotation Biolistics of the flatworm parasite Schistosoma mansoni facilitates the accurate spatial expression of transgenes under the control of gene-specific promoter elements. To improve transgene expression, either in the number of positive worms and/or an increased transgene signal per worm, we tested plasmid constructs incorporating 5_ and 3_ gene-specific genomic fragments, and parts of the open reading frame for two S. mansoni proteases, cathepsins F and D (SmCF and SmCD). GFP-expression was gut-localized, a novel finding for SmCD and consistent with previous data for SmCF. The mCherry fluorescent protein can also operate as a reporter. Though certain constructs imparted stronger and better distributed signals per positive worm, the low yields throughout precluded further quantifications of improvement. Electroporation of the same constructs was also weakly efficient. However, reporter signals were found in tissues other than the gut, which may represent dysregulated transcription. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2011
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