RP LC separation of proteomic samples using simplemicrogradient device in combination with MALDI or ESI mass spectroscopy

0349845 - ÚIACH 2011 AT eng A - Abstract
Řehulka, P. - Kahle, Vladislav - Moravcová, Dana - Řehulková, H. - Lenobel, R. - Sebela, M. - Franc, V. - Hubálek, M. - Hernychová, L.
RP LC separation of proteomic samples using simplemicrogradient device in combination with MALDI or ESI mass spectroscopy.
4th Central and Eastern European Proteomics Conference meets International Metabolomics Austria. Proteomics. Metabolomics. Vienna: Vienna University of Technology, 2010. s. 50.
[Central and Eastern European Proteomics Conference meets Metabolomics Austria /4./. 29.08.2010-03.09.2010, Vienna]
R&D Projects: GA MŠMT LC06023
Institutional research plan: CEZ:AV0Z40310501
Keywords : cell lysate * MS analysis * microgradient device
Subject RIV: CB - Analytical Chemistry, Separation

This work presents a simple microgradient device producing an S-shaped gradient of strong mobile phase for analyte separation using RP capillary column. Separation of the digested cell lysate of pathogenic bacteria Francisella tularensis using this device was compared to the separation of the same sample using a commercial nanoLC instrument with subsequent analysis using on-line connected ESI-Q-TOF instrument.
Permanent Link: http://hdl.handle.net/11104/0189981