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Heterochromatin marks HP1gamma, HP1alpha and H3K9me3, and DNA damage response activation in human testis development and germ cell tumours
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SYSNO ASEP 0347140 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Heterochromatin marks HP1gamma, HP1alpha and H3K9me3, and DNA damage response activation in human testis development and germ cell tumours Author(s) Bartkova, J. (DK)
Moudrý, Pavel (UMG-J)
Hodný, Zdeněk (UMG-J) RID
Lukas, J. (DK)
Rajpert-De Meyts, E. (DK)
Bártek, Jiří (UMG-J) RIDSource Title International Journal of Andrology. - : WILEY-BLACKWELL - ISSN 0105-6263
Roč. 34, 4 Pt 2 (2011), e103-e113Number of pages 11 s. Language eng - English Country GB - United Kingdom Keywords heterochromatinization ; DNA damage response ; germinal tumours Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA301/08/0353 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000293174100013 DOI 10.1111/j.1365-2605.2010.01096.x Annotation Heterochromatinization has been implicated in fundamental biological and pathological processes including differentiation, senescence, ageing and tumorigenesis; however, little is known about its regulation and roles in human cells and tissues in vivo. Here, we show distinct cell-type- and cancer-stage-associated patterns of key heterochromatin marks: histone H3 trimethylated at lysine 9 (H3K9me3) and heterochromatic adaptor proteins HP1α and HP1γ, compared with the γH2AX marker of endogenously activated DNA damage response (DDR) and proliferation markers in normal human foetal and adult testes, pre-invasive carcinoma in situ (CIS) lesions and a series of overt germ cell tumours, including seminomas, embryonal carcinomas and teratomas. Among striking findings were high levels of HP1γ in foetal gonocytes, CIS and seminomas; enhanced multimarker heterochromatinization without DDR activation in CIS; and enhanced HP1α in teratoma structures with epithelial and neuronal differentiation. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2012
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