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The transcription factor EGR1 regulates metastatic potential of v-src transformed sarcoma cells
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SYSNO ASEP 0347089 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The transcription factor EGR1 regulates metastatic potential of v-src transformed sarcoma cells Author(s) Čermák, Vladimír (UMG-J)
Kosla, Jan (UMG-J) RID
Plachý, Jiří (UMG-J) RID
Trejbalová, Kateřina (UMG-J) RID
Hejnar, Jiří (UMG-J) RID
Dvořák, Michal (UMG-J) RIDSource Title Cellular and Molecular Life Sciences - ISSN 1420-682X
Roč. 67, č. 20 (2010), s. 3557-3568Number of pages 12 s. Language eng - English Country CH - Switzerland Keywords smooth muscle cells ; endothelial kinase ; Rho kinase Subject RIV EB - Genetics ; Molecular Biology R&D Projects KAN200520801 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) LC06061 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA204/07/1030 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000282045900013 DOI 10.1007/s00018-010-0395-6 Annotation To advance our understanding of metastatic cancer dissemination, we have developed a model system that is based on two v-src transformed chicken sarcoma cell lines-the highly metastatic parental PR9692 and a non-metastasizing but fully tumorigenic clonal derivative PR9692-E9. Oligonucleotide microarray analysis of both cell lines revealed that the gene encoding the transcription factor EGR1 was downregulated in the non-metastatic PR9692-E9 cells. Further investigation demonstrated that the introduction of exogenous EGR1 into PR9692-E9 cells restored their metastatic potential to a level indistinguishable from parental PR9692 cells. Microarray analysis of EGR1 reconstituted cells revealed the activation of genes that are crucial for actin cytoskeleton contractility (MYL9), filopodia formation (MYO10), the production of specific extracellular matrix components (HAS2, COL6A1-3) and other essential pro-metastatic abilities. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2011
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