Direct voltammetric analysis of DNA modified with enzymatically incorporated 7-deazapurines

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SYSNO ASEP	0346582
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Direct voltammetric analysis of DNA modified with enzymatically incorporated 7-deazapurines
Author(s)	Pivoňková, Hana (BFU-R)_ORCID Horáková Brázdilová, Petra (BFU-R) Fojtová, Miloslava (BFU-R)_{RID, ORCID} Fojta, Miroslav (BFU-R)_{RID, ORCID}
Number of authors	4
Source Title	Analytical Chemistry. - : American Chemical Society - ISSN 0003-2700 Roč. 82, č. 16 (2010), s. 6807-6813
Number of pages	7 s.
Language	eng - English
Country	US - United States
Keywords	modified DNA ; 7-deazapurines ; voltammetric analysis
Subject RIV	BO - Biophysics
R&D Projects	IAA400040901 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	LC06035 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50040507 - BFU-R (2005-2011)
	AV0Z50040702 - BFU-R (2007-2013)
UT WOS	000280758400010
DOI	10.1021/ac100757v
Annotation	Nucleic acids studies use 7-deazaguanine (G) and 7-deazaadenine (A) as analogues of natural purine bases. We show that enzymatic incorporation of G* can easily be monitored by simple ex situ voltammetric analysis of the modified DNA at carbon electrodes. G* is shown as an attractive electroactive marker for DNA, efficiently incorporable by PCR.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2011

Number of the records: 1