New applications of pHluorin - measuring intracellular pH of prototrophic yeasts and determining changes in the buffering capacity of strains with affected potassium homeostasis

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SYSNO ASEP	0345607
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	New applications of pHluorin - measuring intracellular pH of prototrophic yeasts and determining changes in the buffering capacity of strains with affected potassium homeostasis
Author(s)	Marešová, Lydie (FGU-C) Hošková, B. (CZ) Urbánková, E. (CZ) Chaloupka, R. (CZ) Sychrová, Hana (FGU-C)_{RID, ORCID}
Source Title	Yeast - ISSN 0749-503X Roč. 27, č. 6 (2010), s. 317-325
Number of pages	9 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	pH maintenance ; Saccharomyces cerevisiae ; fluorescence measurements
Subject RIV	ED - Physiology
R&D Projects	LC531 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	KJB500110701 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z50110509 - FGU-C (2005-2011)
UT WOS	000279060200002
DOI	10.1002/yea.1755
Annotation	We constructed a new pHluorin plasmid with the dominant selection marker (KanMX). This plasmid allows pH measurements in cells without auxotrophic mutations and/or grown in chemically indefinite media. We also utilised pHluorin in a new method of measuring the buffering capacity of yeast cell cytosol in vivo
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2011

Number of the records: 1