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Probing plant membranes with FM dyes: tracking, dragging or blocking?
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SYSNO ASEP 0343962 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Probing plant membranes with FM dyes: tracking, dragging or blocking? Author(s) Jelínková, Adriana (UEB-Q) RID, ORCID
Malínská, Kateřina (UEB-Q) RID, ORCID
Simon, Sibu (UEB-Q) ORCID
Kleine-Vehn, J. (DE)
Pařezová, Markéta (UEB-Q)
Pejchar, Přemysl (UEB-Q) RID, ORCID
Kubeš, Martin (UEB-Q) RID, ORCID
Martinec, Jan (UEB-Q) RID, ORCID
Friml, J. (DE)
Zažímalová, Eva (UEB-Q) RID, ORCID
Petrášek, Jan (UEB-Q) RID, ORCIDSource Title Plant Journal. - : Wiley - ISSN 0960-7412
Roč. 61, č. 5 (2010), s. 883-892Number of pages 10 s. Language eng - English Country GB - United Kingdom Keywords FM 4-64 ; FM 1-43 ; plasma membrane Subject RIV ED - Physiology R&D Projects KJB600380604 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) LC06034 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000274805600014 DOI 10.1111/j.1365-313X.2009.04102.x Annotation Remarkable progress in various techniques of in vivo fluorescence microscopy has brought an urgent need for reliable markers for tracking cellular structures and processes. The goal of this manuscript is to describe unexplored effects of the FM (Fei Mao) styryl dyes, which are widely used probes that label processes of endocytosis and vesicle trafficking in eukaryotic cells. Although there are few reports on the effect of styryl dyes on membrane fluidity and the activity of mammalian receptors, FM dyes have been considered as reliable tools for tracking of plant endocytosis. Using plasma membrane-localized transporters for the plant hormone auxin in tobacco BY-2 and Arabidopsis thaliana cell suspensions, we show that routinely used concentrations of FM 4-64 and FM 5-95 trigger transient re-localization of these proteins, and FM 1-43 affects their activity. The active process of re-localization is blocked neither by inhibitors of endocytosis nor by cytoskeletal drugs. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2011
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