Mitochondrial membrane potential-based genome-wide RNAi screen of Trypanosoma brucei

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SYSNO ASEP	0342948
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Mitochondrial membrane potential-based genome-wide RNAi screen of Trypanosoma brucei
Author(s)	Verner, Zdeněk (BC-A) Paris, Zdeněk (BC-A)_RID Lukeš, Julius (BC-A)_{RID, ORCID}
Source Title	Parasitology Research. - : Springer - ISSN 0932-0113 Roč. 106, č. 5 (2010), s. 1241-1244
Number of pages	4 s.
Language	eng - English
Country	DE - Germany
Keywords	GENE-FUNCTION ; INTERFERENCE ; mitochondrion ; SUBUNITS
Subject RIV	EB - Genetics ; Molecular Biology
CEZ	AV0Z60220518 - PAU-O, BC-A (2005-2011)
UT WOS	000276234300030
DOI	10.1007/s00436-010-1754-9
Annotation	We have screened the Trypanosoma brucei genome-wide RNAi library by staining the procyclics with the dye JC-1 followed by sorting the differentially stained cells by flow cytometry. This allowed us to highly enrich for cells in which mitochondrial membrane potential was decreased. We have further validated a subset of selected clones by a reverse approach in which we showed that cloning the selected genomic regions into another RNAi vector also results in a drop in mitochondrial membrane potential.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2011

Number of the records: 1