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Detection of condensin I and II in maturing pig oocytes
- 1.0341856 - ÚŽFG 2011 RIV AU eng J - Journal Article
Lišková, Lucie - Šušor, Andrej - Pivoňková, Kateřina - Šašková, Adéla - Karabínová, Pavla - Kubelka, Michal
Detection of condensin I and II in maturing pig oocytes.
Reproduction Fertility and Development. Roč. 22, č. 4 (2010), s. 644-652. ISSN 1031-3613. E-ISSN 1448-5990
R&D Projects: GA ČR GA204/06/1297; GA ČR(CZ) GD204/09/H084; GA ČR GP524/09/P435
Institutional research plan: CEZ:AV0Z50450515
Keywords : Chromosome condensation * Female meiosis * Phosphorylation
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.553, year: 2010
The multiprotein complexes known as condensins (I and II) are major players in chromosome dynamics in mitotic and meiotic cells. Here, we report for the first time the detection of different condensin subunits from both complexes in mammalian oocytes. Using immunoblotting analysis we examined expression levels of condensin subunits during meiotic maturation of porcine oocytes. The expression of the core subunit structural maintenance of chromosomes 2 (SMC2), identical in both condensin complexes, did not change significantly during maturation. Similarly, there was no significant change in the expression of the chromosome associated protein (CAP)-H and CAP-H2 subunits, components of condensin I and II, respectively. Conversely, the expression profiles of CAP-G, CAP-D2 (condensin I) and CAP-D3 (condensin II) were more interesting. At least two isoforms of the CAP-D2 subunit were detected, along with three isoforms of the CAP-D3 and CAP-G subunits.
Permanent Link: http://hdl.handle.net/11104/0184715
Number of the records: 1