Cultivated and wild growing forage crops – reservoirs of viruses and phytoplasmas

0341287 - BC 2010 RIV CZ eng O - Others
Fránová, Jana - Petrzik, Karel - Jakešová, H. - Bečková, M. - Sarkisova, Tatiana
Cultivated and wild growing forage crops – reservoirs of viruses and phytoplasmas.
2009
R&D Projects: GA MZe QH71145
Institutional research plan: CEZ:AV0Z50510513
Keywords : symptoms * electron microscopy * biological and molecular identification
Subject RIV: EE - Microbiology, Virology

Symptoms of mosaic, yellowing/reddening, phyllody, necrosis and dwarf have been observed in cultivated as well as wild growing forage crops. Red clover mottle virus (RCMV, Comovirus), clover yellow vein virus (Potyvirus), potato virus X, white clover mosaic virus (WClMV, Potexvirus), alfalfa latent and pea streak viruses (Carlavirus), new members of genus Cytorhabdovirus and Carlavirus were identified by sequencing. Bacilliform particles (ca 213 – 533 nm by 44 – 58 nm) of the genus Cytorhabdovirus, WClMV, alfalfa mosaic virus (Alfamovirus) and particles resembling members of the genus Badnavirus were observed on negatively stained preparations and ultrathin sections by electron microscopy and mechanically transmitted to host plants. WClMV, RCMV, bean yellow mosaic virus and new member of Potyviridae family were determined in wild growing plants. The occurrence of phytoplasmas belonging to different 16S ribosomal subgroups was confirmed (16SrI-B, 16SrI-C, 16SrX-A, 16SrXII-A).
Permanent Link: http://hdl.handle.net/11104/0184315