Activity of the phenol catabolic enzymes from Rhodococcus erythropolis

0340755 - MBÚ 2010 RIV CH eng A - Abstract
Knoppová, Monika - Szököl, Juraj - Halada, Petr - Nešvera, Jan - Čejková, A. - Masák, J. - Jirků, V. - Pátek, Miroslav
Activity of the phenol catabolic enzymes from Rhodococcus erythropolis.
Biotrans 2009. Bern: Springer, 2009. s. 23-23.
[International Symposium on Biocatalysis /9./. 05.07.2009-09.07.2009, Bern]
R&D Projects: GA MŠMT 2B08062
Institutional research plan: CEZ:AV0Z50200510
Keywords : phenol
Subject RIV: EE - Microbiology, Virology

Induction of the phenol hydroxylase and catechol dioxygenase activity with phenol was suppressed by succinate in the growth medium, whereas presence of glucose did not influence it. The cluster of genes responsible for phenol degradation, pheA2A1RcatRABC, was cloned and sequenced. To improve the phenol-catabolic activity, recombinant strains R. erythropolis (pSRKcatRABC), R. erythropolis (pSRKpheA2A1R) and R. erythropolis catR were constructed. In all cases, the constructed strains degraded phenol faster than the WT-strain
Permanent Link: http://hdl.handle.net/11104/0183934