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Cloning and Molecular Analysis of HlbZip1 and HlbZip2 Transcription Factors Putatively Involved in the Regulation of the Lupulin Metabolome in Hop (Humulus lupulus L.)
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SYSNO ASEP 0340099 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Cloning and Molecular Analysis of HlbZip1 and HlbZip2 Transcription Factors Putatively Involved in the Regulation of the Lupulin Metabolome in Hop (Humulus lupulus L.) Author(s) Matoušek, Jaroslav (BC-A) RID, ORCID
Kocábek, Tomáš (BC-A) RID, ORCID
Patzak, J. (CZ)
Stehlík, Jan (BC-A)
Füssy, Zoltán (BC-A) RID, ORCID
Krofta, K. (CZ)
Heyerick, A. (BE)
Roldán-Ruiz, I. (BE)
Maloukh, L. (BE)
De Keukeleire, D. (BE)Number of authors 10 Source Title Journal of Agricultural and Food Chemistry. - : American Chemical Society - ISSN 0021-8561
Roč. 58, č. 2 (2010), s. 902-912Number of pages 11 s. Language eng - English Country US - United States Keywords secondary metabolites transcriptional regulation ; cDNA-AFLP analysis ; hop cDNA library screening ; Nicotiana benthamiana ; Petunia hybrida Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA521/08/0740 GA ČR - Czech Science Foundation (CSF) QH81052 GA MZe - Ministry of Agriculture (MZe) ME 940 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50510513 - UMBR-M, BC-A (2005-2011) UT WOS 000273671900032 DOI 10.1021/jf9043106 Annotation TFs HlbZIP1A and HlbZIP2 cloned from a cDNA library based on glandular tissue-enriched hop cones are similar to the group A3 bZIP TFs according to characteristic domains. While HlbZIP1A is rather neutral (pl 6.42), HlbZIP2 is strongly basic (pl 8,51). Both bZIP TFs show specific expression in lupulin glands, although low expression was observed also in roots and immature pollen. In a transient expression system, both hop bZIP TFs activated the GUS-coupled promoters of chalcone synthase chs_H1 and O-methyl transferase 1, while the response of the valerophenone synthase promoter to the cloned hop TFs was very low, showing that the cloned bZIP TFs are not strictly G-box-specific. HPLC analysis of secondary metabolites in infiltrated Petunia hybrida showed that both bZIPs interfere with the accumulation and the composition of flavonol glycosides, phenolic acids, and anthocyanins, suggesting the possibility of coregulating flavonoid biosynthetic pathways in hop glandular tissue. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2010
Number of the records: 1