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Antitumor Effects and Cytotoxicity of Recombinant Plant Nucleases
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SYSNO ASEP 0340098 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Antitumor Effects and Cytotoxicity of Recombinant Plant Nucleases Author(s) Matoušek, Jaroslav (BC-A) RID, ORCID
Podzimek, Tomáš (BC-A)
Pouckova, P. (CZ)
Stehlík, Jan (BC-A)
Škvor, J. (CZ)
Souček, J. (CZ)
Matoušek, Josef (UZFG-Y) RIDNumber of authors 7 Source Title Oncology Research - ISSN 0965-0407
Roč. 18, č. 4 (2009), s. 163-171Number of pages 9 s. Language eng - English Country US - United States Keywords Anticarcinogenic and antiproliferative nucleases ; Human melanoma ; Tumor xenografts ; Nicotiana benthamina Subject RIV FD - Oncology ; Hematology R&D Projects GA521/09/1214 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50510513 - UMBR-M, BC-A (2005-2011) AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000272875000004 Annotation Antitumor effects and cytotoxicity of recombinant plant nucleases R-TBN1 and R-HBN1 were studied. The nucleases were strongly cytostatic in vivo after their administration intravenously as stabilized conjugates with polyethyleneglycol. Both nucleases were as effective against human melanoma tumors grown on mouse model as previously studied pine pollen (PN) and mung bean nucleases and their effects were reached at about 10 times lower concentrations compared to bovine seminal RNase (BS-RNase). Because R-HBN1 and R-TBN1 share only 67.4% amino acid identity and only partial immunochemical cross-reactivity, their similar anticancerogenic effects can be explained by their catalytical similarity. Both recombinant nucleases showed lower degree of aspermatogenesis compared to BS-RNase and PN nuclease. Owing to low cytotoxicity on the one hand, and high efficiency on the other, R-HBN1 and R-TBN1 appear to be stable biochemical agents that can be targeted as potential antitumor cytostatics. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2010
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