Detection and sequence comparison of the Blackcurrant reversion virus in black, red and white currant

0339363 - BC 2010 RIV BE eng C - Conference Paper (international conference)
Přibylová, Jaroslava - Špak, Josef - Petrzik, Karel - Kubelková, Darina - Špaková, Vlastimila
Detection and sequence comparison of the Blackcurrant reversion virus in black, red and white currant.
Proceedings of the eleventh international symposium on small fruit virus diseases. Leueven 1: ISHS Acta Horticulturea, 2008 - (Martin, R.), s. 61-65. ISBN 978-90-6605-038-9. ISSN 0567-7572.
[International Symposium on Small Fruit Virus Diseases /11./. Antalya (TR), 22.05.2006-26.05.2006]
Institutional research plan: CEZ:AV0Z50510513
Keywords : Blackcurrant reversion virus * curran * RT-PCR
Subject RIV: EF - Botanics

We collected samples from black, red and white currants (Ribes spp.) showing symptoms of blackcurrant reversion disease (severe form) and full blossom disease (FBD), cultivated in the Czech Republic. We detected 15 new isolates of the Blackcurrant reversion virus (BRV) with RT-PCR and two sets of primers (Lemmetty et al., 1998, 2001). After amplification, a substantial part of the 3' untranslated region (UTR) of BRV RNA 2 was sequenced and analyzed. The nucleotide sequence identity among the Czech isolates ranged from 91.9 to 99.8%.
Permanent Link: http://hdl.handle.net/11104/0182912