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Glucose determination using a re-usable enzyme-modified ion track membrane sensor
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SYSNO ASEP 0338731 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Glucose determination using a re-usable enzyme-modified ion track membrane sensor Author(s) Fink, Dietmar (UJF-V) ORCID, SAI
Klinkovich, I. (IL)
Bukelman, O. (IL)
Marks, R.S. (IL)
Kiv, A. (IL)
Fuks, D. (IL)
Fahrner, W. R. (DE)
Alfonta, L. (IL)Source Title Biosensors and Bioelectronics. - : Elsevier - ISSN 0956-5663
Roč. 24, č. 8 (2009), s. 2702-2706Number of pages 5 s. Language eng - English Country GB - United Kingdom Keywords Glucose sensor ; etched tracks ; Ion track membranes Subject RIV BG - Nuclear, Atomic and Molecular Physics, Colliders CEZ AV0Z10480505 - UJF-V (2005-2011) UT WOS 000265811000064 DOI 10.1016/j.bios.2008.12.001 Annotation A novel concept for a glucose biosensor based on the enzyme glucose oxidase covalently linked to nanopores of etched nuclear track membranes is presented. This device can be used to detect physiologically relevant glucose concentrations between 10 mu M and 1 M. The sensitive catalytic sensor can be made re-usable due to the production of diffusible products from the oxidative biomolecular recognition event. We have demonstrated both the simplicity with which this sensor can be produced and the stability of the enzyme embedded in the nanopores. We strongly believe that the approach could be expanded for different enzymes and variable configurations of sensing and catalysis using membranes with nanopores for biocatalysis and enrichment of substrates and products. This is the first time that such devices are being used for biocatalytic transformations. Workplace Nuclear Physics Institute Contact Markéta Sommerová, sommerova@ujf.cas.cz, Tel.: 266 173 228 Year of Publishing 2010
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