A novel mosquitocidal Bacillus thuringiensis strain LLP29 isolated from the phylloplane of Magnolia denudata

0337494 - BC 2010 RIV DE eng J - Journal Article
Zhang, L. - Huang, E. - Lin, J. - Gelbič, Ivan - Zhang, Q. - Guan, Y. - Huang, T. - Guan, X.
A novel mosquitocidal Bacillus thuringiensis strain LLP29 isolated from the phylloplane of Magnolia denudata.
Microbiological Research. Roč. 165, č. 2 (2010), s. 133-141. ISSN 0944-5013. E-ISSN 1618-0623
R&D Projects: GA MŠMT 2B08003
Grant - others:United Fujian Provincial Health and Education Project for Tackling Key Research(CN) WKJ2008-2-44; Talented Youth Project of Fujian Province(CN) 2008F3012; Educational Department of Fujian Province(CN) JA08080; Fujian Agriculture and Forestry University(CN) 08A01
Institutional research plan: CEZ:AV0Z50070508
Keywords : Bacillus thuringiensis * cyt1 * mosquito
Subject RIV: GF - Plant Pathology, Vermin, Weed, Plant Protection
Impact factor: 1.958, year: 2010

Eleven Bacillus thuringiensis isolates were recovered from phylloplanes of Magnolia denudata, a specific source of new strains of B. thuringiensis. Among these, a new strain, LLP29, was found to be most toxic to mosquitoes based on the results of preliminary toxicity analysis. Phase contrast microscopy, mosquitocidal activity, polymerase chain reaction (PCR) analysis and parasporal inclusion were performed to learn more about the characteristics of his novel mosquitocidal isolate. The LC50 values of LLP29 against Aedes albopictus and Culex quinquefasciatus were 0.33 and 0.04 ng of protein/ml, respectively. The cyt1 gene, which encodes the Cyt protein that is toxic to mosquitoes, was subsequently detected, cloned, sequenced and expressed in a crystalliferous Bt HD73Cry_. The results indicated that it might be a member of the cyt1Aa gene group.
Permanent Link: http://hdl.handle.net/11104/0181473