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Silencing CENPF in bovine preimplantation embryo induces arrest at 8-cell stage

  1. 1.
    SYSNO ASEP0334003
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleSilencing CENPF in bovine preimplantation embryo induces arrest at 8-cell stage
    Author(s) Toralová, Tereza (UZFG-Y) ORCID
    Šušor, Andrej (UZFG-Y) RID, ORCID
    Němcová, Lucie (UZFG-Y) RID
    Kepková, Kateřina (UZFG-Y)
    Kaňka, Jiří (UZFG-Y) RID, ORCID
    Source TitleReproduction. - : BioScientifica - ISSN 1470-1626
    Roč. 138, č. 5 (2009), s. 783-791
    Number of pages9 s.
    Languageeng - English
    CountryGB - United Kingdom
    KeywordsCENPF ; bovine embryo ; preimplantation development
    Subject RIVGI - Animal Husbandry ; Breeding
    R&D ProjectsGA523/06/1226 GA ČR - Czech Science Foundation (CSF)
    GD204/05/H023 GA ČR - Czech Science Foundation (CSF)
    CEZAV0Z50450515 - UZFG-Y (2005-2011)
    UT WOS000271572000004
    DOI10.1530/REP-09-0234
    AnnotationThe paper deals with the role of CENPF/mitosin during preimplantation development of cattle. We focused on monitoring of developmental competence of bovine embryos after injection of CENPF dsRNA into the zygotes. An average decrease of CENPF mRNA abundance by 94.9% or more and an extensive decline in immunofluorescence staining intensity was detected relative to controls. There was no disparity between the groups in the developmental competence before the 8-cell stage, where embryonic genome activation (EGA) in bovines takes place. However, the developmental competence rapidly decreased then and only 28.1% of CENPF dsRNA injected 8-cell embryos were able to develop further (controls 72.0%). We have further shown that in bovine post-EGA embryos CENPF protein is expressed in the same manner as in somatic cells. However, the protein in pre-EGA embryos is likely not degraded at the end of cell cycle, which enables the development of embryos till 8-cell stage.
    WorkplaceInstitute of Animal Physiology and Genetics
    ContactJana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
    Year of Publishing2010
Number of the records: 1  

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