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Silencing CENPF in bovine preimplantation embryo induces arrest at 8-cell stage
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SYSNO ASEP 0334003 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Silencing CENPF in bovine preimplantation embryo induces arrest at 8-cell stage Author(s) Toralová, Tereza (UZFG-Y) ORCID
Šušor, Andrej (UZFG-Y) RID, ORCID
Němcová, Lucie (UZFG-Y) RID
Kepková, Kateřina (UZFG-Y)
Kaňka, Jiří (UZFG-Y) RID, ORCIDSource Title Reproduction. - : BioScientifica - ISSN 1470-1626
Roč. 138, č. 5 (2009), s. 783-791Number of pages 9 s. Language eng - English Country GB - United Kingdom Keywords CENPF ; bovine embryo ; preimplantation development Subject RIV GI - Animal Husbandry ; Breeding R&D Projects GA523/06/1226 GA ČR - Czech Science Foundation (CSF) GD204/05/H023 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000271572000004 DOI 10.1530/REP-09-0234 Annotation The paper deals with the role of CENPF/mitosin during preimplantation development of cattle. We focused on monitoring of developmental competence of bovine embryos after injection of CENPF dsRNA into the zygotes. An average decrease of CENPF mRNA abundance by 94.9% or more and an extensive decline in immunofluorescence staining intensity was detected relative to controls. There was no disparity between the groups in the developmental competence before the 8-cell stage, where embryonic genome activation (EGA) in bovines takes place. However, the developmental competence rapidly decreased then and only 28.1% of CENPF dsRNA injected 8-cell embryos were able to develop further (controls 72.0%). We have further shown that in bovine post-EGA embryos CENPF protein is expressed in the same manner as in somatic cells. However, the protein in pre-EGA embryos is likely not degraded at the end of cell cycle, which enables the development of embryos till 8-cell stage. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2010
Number of the records: 1